Changes and their clinic significan ce of S-100B and myelin basic protein in serum and cerebrospinal fluid after acute spinal cord injury and after te tramethylpyrazine treatment / 中华创伤骨科杂志

Objective To explore changes of myelin basic pr otein(MBP)and S-100B protein in serum and cerebrospinal fluid(CSF)after acute spinal cord injury(ASCI )and after tetramethylpyrazine trea tment.Methods Dog models with ASCI at T13were made in th e methods of Allens and an epidural t ube was set in the subarachnoid space,with one opening out of the skin and blocked in order to gather CSF.The dogs were divided randomly into 3groups.Grou p A(n =6)was the control group which received only laminectomy of T13without a str iking to the spinal cord.Group B(n =6),acute spinal cord injury group and Group C(n =6),tetramethylpyrazine treatment group,all received a striking to the spinal cord with the device of Allens after the dura was exposed.Blood an d CSF were taken out of the dogs respec tively at the time intervals of 2h,4h,6h,8h,10h,24h,48h,72h and 96h after the operation.The concentration of MBP and S-100B in the serum and CSF was measur ed by the methods of ELISA and feature s of the change were studied.Results After acute spinal cord injury,concentrations of MBP and S-100B in the se rum and CSF increased significantly in Group B and Group C,and the levels changed dynamically.But the levels in tetrame thylpyrazine treatment group were l ower than those in spinal cord injury group.Conclusions MBP and S-100B protein change dynami cally after acute spinal cord injury.Tetramethylpyrazine has a t herapeutic effect on acute spinal cord injury.Whether MBP and S-100B pro tein can be served as the marker for ASCI needs fu rther research to evaluate their correlation to the severity and prognosis of ASCI.[